UNAIR NEWS – Five Biomedical Engineering students of Faculty of Science andersitasAirlangga, Evlyn Anggraini Santoso, Fulky A’yunni, Fathania Nabilla, Novita Putri Rahayu, and Adita Wardani Rahmania have made an innovation through their research aboutdialyzermembranes (membranes for dialysis / Hemodialysis) with antibacterial properties.
Hemodialysistherapy(HD) is the most selected treatment for patients with chronic renal failure.听Based on a survey of various centers ofhemodialysis, in Indonesia there are 30.7% of patients per million inhabitants who requirehemodialysis.听Routine vascular accessed during every HD procedure can lead to bacteriological conditions and infections that can degrade the patient’s quality of life and bring death.听Besides vascular access, bacterial infections can result from dialysis fluid, reused dialyzer, water treatment at dialysis centers and dialysis machines.
Thedialyzermembranesused so far have no antibacterial properties when there are many external factors from the process of HD treatment which can cause infection, so the albumin decreases.听The percentage of infection occurring within a month by 15%, 1-12 months 44%, and above 1 year 41%.听The occurrence of this infection is caused by gram-positive pathogens of 97% of cases, mainly byStaphylococcus aureus(60%) andStaphylococcus epidermis听(22%).
Therefore, under the guidance of Dr.听Prihartini Widiyanti, drg., M.Kes., The student is trying to make adialyzer听尘别尘产谤补苍别蝉听蹿辞谤听hemodialysistherapythat has antibacterial properties, so as to improve the quality of life of patients with kidney failure.
“We tried to make a membranesdialyzermade ofpolyurethane听,听citric acid听补苍诲听polyethersulfonto later added chitosan to elicit its antibacterial properties.听Chitosan is a natural ingredient known to have high antibacterial properties, is easy to find, and the price is not too expensive, “said Evlyn Anggraini Santoso, group leader.
惭别尘产谤补苍别蝉听dialyzerwhich has been printed in two formsflat(flat) andhollow fiberis then dipped in a solution of chitosan and stored for 24 hours at 4 掳 C.听After it is washed using PBS solution and sterilization is done with soaked in glutaraldehyde for 30 minutes.
Research is packaged in the title “biocomposite Innovative Based Citric Acid-polyurethane-Chitosan as a candidate MembranessDialyzerAntibacterials” managed to attractHigher Education (Higher Education) Kemenristek Higher Education, which then gives the fund the development of research through the Student Creativity Program Research Eksakta (PKM-PE) .
The results of this study have been tested with antibacterial tests using bacteriaStaphylococcus aureusand showed a clear zone around the disc diameter of about 6.946 to 11.086 mm.听This indicates that the sample of thedialyzermembraneshas antibacterial properties.听The antibacterial activity of chitosan derived from amine functional group that can form a bond with the cell walls of bacteria and bacteria lysis.
FTIR test results indicate the discovery of the functional groups of chitosan NH / OH (amine and hydroxyl) which constituent chitosan.听The results of measurements have been carried out using a membranes pore SEM test and obtained 0.944 渭m pore size, these results are in accordance with the pore size of the membranes filtration.
In addition,hemolysistests have been doneto determine the material properties when contacted directly with blood.听If the percentage ofhemolysis isbelow 5% then the sample is considered safe for contact with blood.听From the results of the test the five samples showed percentage ofhemolysisless than 5% so safe to use forhemodialysisprocess.听(*)
Editor: Bambang Bes
