UNAIR NEWS “ Biomedical Engineering students of Faculty of Science and Technology (FST) succeeded in making innovations in the form of barrier membrane that can trigger tissue regeneration for the therapy of periodontal abnormalities.One of the periodontal abnormalities is periodontitis, a disease that causes chronic inflammation due to bacteria causing tooth and gum damage, loss of connective tissue, erosion ofalveolarboneand tooth decay.
“If It is not treated immediately this disease will cause big and systemic problems, such as atherosclerosis, diabetes, heart disease andauto-imune,” said Mohammad Bagus Lazuardi, one of three FST students who joined the Student Creativity Program Exacta Research team (PKM- PE).
With his colleagues, Fitria Renata Bella and Marsya Nilam Kirana, they wrote his research in a proposal titled “MembraneGuided Tissue RegenerationPCL-AgNPsAloe verafor Periodontal Disorder Therapy”.This proposal has passed the selection of Dikti, thus obtaining research grant funds in PKM program Kemenristekdikti 2017-2018.
“As reported by a media, from four people there is one person with dental and mouth problems, so the projection is estimated to be 65.275 million people with dental and mouth problems,” continued M Bagus Lazuardi quoting the results of Basic Health Research (Riskesdas 2013).
So one clinical treatment for periodontitis requires abarriermembrane,or anchoring called theGuided Tissue Regeneration(GTR) which prevents the release of gum tissue(ephitelium)and ensures the growth of ligament cells in damaged periodontal areas.
As we all know, clinical GTR membranes used today usenon-biodegradable polymericmaterials(PTFE), so physically require a second surgical procedure for removal of the membrane, and chemically gives less support for cell growth.
“Failure of healing often occurs due to infection caused by pathogen colonization at the site of periodontitis, or foreign body response.Therefore, it needs a biocompatible and antibacterial GTR membrane, “said M. Bagus Lazuardi, chief of innovation team.
Under the guidance of Dr.Prihartini Widiyanti, drg., M.Kes., The research team is trying to createPoly-based GTR(É›-³¦²¹±è°ù´Ç±ô²¹³¦³Ù´Ç²Ô±ð)(PCL)membranes,nanosilver(AgNPs), and aloe vera (Aloe veraÌý).
“We are trying to make GTR membranes fromPoly (É›-³¦²¹±è°ù´Ç±ô²¹³¦³Ù´Ç²Ô±ð)(PCL), AgNPs, andAloe vera. PCL is safe for the body because it can be degraded, then eliminated from the body through the citric acid cycle, then combined with AgNPs ofAloe verabiosynthesis productsthat are reportedly able to effectively kill periodontitis-causing bacteria, “added M Bagus.
This GTR membrane is a sheet offibermade withelectrospinninginstruments.This GTR membrane has also been tested using FTIR and shows that PCL-AgNPsAloe verais contained in the membrane.Observations under the electron microscope indicate that the diameter of the fibers formed ranges from 106 to 113 nm, thus resembling theextracellular matrix(ECM).
The resulting GTR membrane also has advantages over the current clinical GTR membrane, ie the membrane can be degraded and its degradation rate can be adjusted.The results of this study also escaped from antibacterial tests using bacteriaE. coliandS.aureuswhich is the bacteria that cause early periodontitis.The results of the test, the resulting GTR membrane can inhibit the growth of these bacteria.
M Bagus also said that to be make sure that GTR membrane does not cause foreign body reactions and is toxic to the body, then the cytotoxicity test is done using lymphocyte cells.As a result, the GTR membrane is not toxic, so it is safe for the body.(*)
Editor: Bambang Bes
